Vol. 2024 No. 1 (2024)
Articles

Electrochemical immunosensors for 2,4-dichlorophenoxyacetic acid based on 3-aminopropyltrimethoxysilane modified electrodes

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  • Rui Xue,
  • Tianfang Kang,
  • Xin Rui
Rui Xue
China Electronics Engineering Design Institute, Beijing 100142, P. R. China.
Tianfang Kang
Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, P. R. China
Xin Rui
China Electronics Engineering Design Institute, Beijing 100142, P. R. China

Published 26-01-2024

Keywords

  • Electrochemical Immunosensor,
  • 3-aminopropyltrimethoxysilane,
  • 2,4-Dichlorophenoxyacetic acid

Copyright (c) 2024 Cambridge Science Advance

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
R. Xue, T. Kang, and X. Rui, “Electrochemical immunosensors for 2,4-dichlorophenoxyacetic acid based on 3-aminopropyltrimethoxysilane modified electrodes”, Camb. Sci. Adv., vol. 2024, no. 1, pp. 1–9, Jan. 2024, doi: 10.62852/csa/2024/1.

Abstract

A novel electrochemical immunosensor prepared by direct coating of small molecular hapten on glassy carbon electrode (GCE) is reported for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D). The glassy carbon electrodes were activated by electrochemical method and then were further treated with 3-aminoprpyltriethoxysilane (APS) to functionalize the GCE surface with amino groups for covalent linkage to small molecular hapten of 2,4-D with carboxyl groups. The electrodes were characterized by X-ray photoelectron spectroscopy (XPS). The principle of this immunoassay is based on an indirect competitive immunoassay. Analytes of 2,4-D and hapten of 2,4-D immobilized on glassy carbon electrode (GCE) compete for 2,4-D antibodies labeled by horseradish peroxidase (HRP-anti-2,4-D). After complete immunoreaction, the GCE attached HRP-anti-2,4-D tracers was transferred to a substrate solution containing hydroquinone (QH2) and hydrogen peroxide for square wave voltammetry (SWV) detection. Determination conditions were optimized. The SWV anodic peak current decreased linearly with the increase of 2,4-D concentration over the range from 0.10 to 15.0 mg/L 2,4-D with a detection limit of 0.01mg/L. The performance of this electrochemical immunoassay was successfully evaluated with river water spiked with 2,4-D, indicating that this convenient, rapid, and sensitive technique offers great prospect for the monitoring of trace 2,4-D in environment.

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