Vol. 2025 No. 1 (2025)
Articles

An Identification of B(A)02 subgroup blood

Long Dai
Shanghai Renzhi Medical Research Co., Ltd., Room 178-7, Building 3, No. 1999 Beixing Road, Sanxing Town, Chongming District, Shanghai, 202162, China
Yaze Wang
Shanghai Renzhi Medical Research Co., Ltd., Room 178-7, Building 3, No. 1999 Beixing Road, Sanxing Town, Chongming District, Shanghai, 202162, China
cover image

Published 31-12-2024

Keywords

  • ABO subtypes,
  • B (A) blood group,
  • Alleles,
  • Blood group genotyping

How to Cite

[1]
L. Dai and Y. Wang, “An Identification of B(A)02 subgroup blood”, Camb. Sci. Adv., vol. 2025, no. 1, pp. 1–5, Dec. 2024, doi: 10.62852/csa/2025/111.

Abstract

Objective To explore the necessity and feasibility of RBC blood typing by molecular technique for unidentified blood group. Methods The blood group was identified by serological test and molecular detection. Results One case of blood donor showed weakly positive reaction to anti -A, strongly positive reaction to anti-B and anti -H, weakly positive reaction to A 1 RBCs but no reaction to either B or O blood type. The sample was subjected to the detection of ABO genes and the results showed 9 mutants in Exons 6 and 7. The type B(A)02 was identified when comp a red with A101 allele. Conclusion Genetic detection is helpful for finding B(A) subgroup blood that cannot be identified by serological test.

References

  1. Linden JV, Wagner K, Voytovich AE, et al. Transfusion errors in New York State: an analysis of 10 years' experience [J]. Trans fusion,2000,40(10):1207-1213. DOI: https://doi.org/10.1046/j.1537-2995.2000.40101207.x
  2. Sun Xiaolin, Guan Xiao Zhen, Yu Yang, et al. Detection and serological analysis of 36 cases of ABO blood group subtypes [J]. Clinical Transfusion and Testing, 2012, 14(3): 215-217.
  3. Wang Chao. Discussion on the identification and genotyping of difficult blood types in cancer patients [J]. Chinese Journal of Experimental Hematology, 2013, (3): 780.
  4. Yuan Zhi Chen. Analysis of the causes and countermeasures for the inconsistency of ABO blood group forward and reverse typing [J]. China Practical Medicine, 2012, 7(34): 66-67.
  5. Ma Shu Xuan, Liu Jinghan, Wang Quan li, et al. Serological analysis of 24 cases of ABO blood group forward and reverse typing inconsistency in recipients [J]. Clinical Transfusion and Testing, 2005, 7(4): 280-282.
  6. Ministry of Health of the People's Republic of China. Technical procedures for blood transfusion in China (blood station part) [M]. Tianjin: Tianjin Science and Technology Press, 1997: 62-64.
  7. Jeff Daniel. Zhu Ziyan, trans., Human Blood Types [M]. Beijing: Science Press, 2007: 4546.
  8. Wu Gang, Du Hai lin. Establishment and application of methods for identifying difficult ABO blood types [J]. Clinical Transfusion and Testing, 2015, 17(1): 68-70.
  9. Yamamoto F, McNeill PD, Yamamoto M, et al. Molecular genetic analysis of the ABO blood group system:3. A(x)and B(A)alleles[J]. Vox Sang,1993,64(3):171-174 DOI: https://doi.org/10.1159/000462337
  10. Guo Zhong hui, Xiang Dong, Zhu Ziyan, et al. Detection of a new B(A)641T>C allele in the Chinese Han population [J]. Chinese Journal of Blood Transfusion, 2006, 19(1): 17-20.
  11. Liu Lexia, Liang Junjie, Xiao Wei, et al. Investigation of the inheritance pattern in a rare B(A)04 blood type family [J]. Clinical Transfusion and Testing, 2014, 16(2): 173-176.
  12. Chen Q, Li JH, Xiao JY, et al. Molecular genetic analysis and structure model of a rare B(A) 02 subgroup of the ABO blood group system[J]. Transfusion and Apheresis Science,2014,51(2):203- 208. DOI: https://doi.org/10.1016/j.transci.2014.08.023
  13. Li Huimin, Lin Jun feng, Zhang Fan, et al., Molecular identification of a rare B(A) blood type [J]. Journal of Laboratory Medicine, 2014, 29(9): 945-951.
  14. Zhuang Wenhua, Zhuang Cai Mei. Detection and analysis of cis AB and Ax B blood types [J]. Clinical Transfusion and Testing, 2014, 16(4): 434-435.