Vol. 2025 No. 3 (2025)
Articles

Application of silk fibroin in biomedical engineering

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  • Takumi Saito
Takumi Saito
Graduate School of Medicine, Kyoto University,53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto,606-8507, Japan

Published 04-06-2025

Keywords

  • Silk fibroin,
  • Biomedicine,
  • Biomedical engineering,
  • Application

Copyright (c) 2025 Cambridge Science Advance

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
T. Saito, “Application of silk fibroin in biomedical engineering”, Camb. Sci. Adv., vol. 2025, no. 3, pp. 6–12, Jun. 2025, doi: 10.62852/csa/2025/153.

Abstract

Silk fibroin is the main component of silk and is known for its excellent biocompatibility, degradability, mechanical properties, and safety. It is non-toxic, and its raw materials are readily available, making it a popular material in the preparation and research of biomedical materials. This article reviews the healthcare benefits of silk fibroin, such as its role in lowering blood sugar and inhibiting bacterial growth, as well as its applications and development prospects in biomedical engineering fields, including drug release, hemostasis, wound healing, tissue engineering scaffolds, and other biomedical materials.

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References

  1. LD Koh, Y Cheng, CP Teng, et al. Structures, mechanical properties and applications of silk? fibroin? materials [J] Progress in Polymer Science,2015,46(7):86-110.
  2. H Mori, M Tsukada. New silk protein: Modification of silk protein by gene engineering for production of bio- materials[J]. Journal of Biotechnology,2000,74(2):95-103.
  3. CK Hyun, IY Kim, SC Frost. Soluble fibroin enhances insulin sensitivity and glucose metabolism in 3T3-L1 adipocytes [J]. Journal of Nutrition,2005,134(12):3257-3263.
  4. JH Park, YY Nam, SY Park, et al. Silk fibroin has a protective effect against high glucose induced apoptosis in HIT-T15 cells [J]. Journal of Biochemical &Molecular Toxicology, 2011,25(4): 238-243.
  5. Jin Hanyu, Liu Xing, Yin Hua, et al. Application of silk fibroin in the biomedical field[J]. Journal of Biomedical Engineering,2014,31(1):228-232.
  6. Li Ya Jie, Wang Lin Hua, Shi Lixin, et al. Study on the antibacterial action of oak silk moth and domestic silk moth silk peptides[J]. Liaoning Silk,2005(3):7-9.
  7. Takeuchi, Shigeru. Enzymic manufacture of low-mole cular weight silk fibroin peptides[J]. J p n. Kokai Tokkyo Ko- ho JP,1995(7):670-686.
  8. Cao Yang, Wang Bo chu, Chi Shao ping, et al. Drug release materials based on silk fibroin[J]. Chinese Journal of Tissue Engineering Research and Clinical Rehabilitation,2009,13(8):1533-1536.
  9. E Wenk, HP Merkle, L Meinel. Silk fibroin as a vehicle for drug delivery applications [J]. J Control Release, 2010,150(2):128-141.
  10. K Lerd chai, J Kitsongsermthon, J Ratanavaraporn, et al. Thai silk fibroin/gelatin dponges for the dual controlled release of curcumin and docosahexaenoic acid for anticancer treatment [J]. Journal of Pharmaceutical Sciences, 2015,105(1):221-230.
  11. Xu Ya yang, Shen Jinyu, Zhu Hai Lin, et al. Morphological structure and hemostatic properties of silk fibroin peptides with different molecular weights[J]. Sericultural Science,2015,41(2):354-360.
  12. Zhu Li Bing, Dong Li, Han Sumei. Development and application of silk fibroin in the medical and health fields[J]. Taishan Health,2001,25(6):33.
  13. S Min, X Gao, C Han, et al. Preparation of a silk fibroin spongy wound dressing and its therapeutic efficiency in skin defects[J]. Journal of Biomaterials Science Polymer Edition, 2012, 23(1-4):97-110.
  14. A Vasconcelos, AC Gomes, A Cavaco-Paulo. Novel silk fibroin /elastin wound dressings [J]. Acta Biomaterialia, 2012,8(8):3049-3060.
  15. Z Gu, HX Xie, C Huang, et al. Preparation of chitosan /silk fibroin blending membrane fixed with alginate dialdehyde for wound dressing [J]. International Journal of Biological Macromolecules, 2013,58(7):121-126.
  16. Zhang Jian, Dou Yi, Liao Zhenjiang. The effect of silk fibroin dermal scaffold on the function of fibroblasts during wound healing[J]. Infection, Inflammation, and Repair,2015,16(4):195-198.
  17. R Okabayashi, M Nakamura, T Okabayashi, et al. Efficacy of polarized hydroxyapatite and silk fibroin composite dressing gel on epidermal recovery from full-thickness skin wounds[J]. Journal of Biomedical Materials Research Part B Applied Biomaterials,2009,90(2): 641-646.
  18. DH Roh, SY Kang, JY Kim, et al. Wound healing effect of silk fibroin alginate blended sponge in full thickness skin defect of rat [J]. Journal of Materials Science Materials in Medicine, 2006, 17(6):547-552.
  19. HW Ju, OJ Lee, JM Lee, et al. Wound healing effect of electrospun silk fibroin nanomatrix in burn-model[J]. International Journal of Biological Macromolecules, 2016,85:29-39.
  20. SE Wharram, X Zhang, DL Kaplan, et al. Electrospun silk material systems for wound healing[J]. Macromolecular Bioscience,2010,10(3):246-257.
  21. Cai Z X, Mo XM, Zhang K H, et al. Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications [J]. International Journal of Molecular Sciences, 2010, 11(9):3529-3539.
  22. Schneider A, Wang XY, Kaplan D L, et al. Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing [J]. Acta Biomaterialia,2009,5(7):2570-2578.
  23. Inpanya P, Faikrua A, Ounaroon A, et al. Effects of the blended fibroin /aloe gel film on wound healing in treptozotoci induced diabetic rats [J]. Biomed Mater, 2012,7(3):35-38.
  24. Xu Zong p u, Yang Ming ying, Pan Caixia, et al. Advances in the preparation and application of silk fibroin wound dressings[J]. Sericultural Science,2015,41(2):376-380.
  25. Sun Hao, Yan Yusheng, Chen Qun Qing, et al. Application of silk fibroin in small-diameter artificial blood vessels[J]. Chinese Journal of Tissue Engineering Research,2014,18(16):2576-2581.
  26. Shi Li yang, Yang Ming ying, NAMITA Mandal, et al. Advances in the application of silk fibroin biomaterials in bone repair[J]. Sericultural Science,2013,39(4):812-819.
  27. Guan G, Bai L, Zuo B, et al. Promoted dermis healing from full-thickness skin defect by porous silk fibroin scaffolds (PSFSs)[J]. Biomedical materials and engineering,2010,20(5):295-308.
  28. Uebersax L, Mattotti M, Papaloizos M, et al. Silk fibroin matrices for the controlled release of nerve growth factor (NGF)[J]. Biomaterial,2007,28(30):4449-4460.
  29. Zhu Chen Hui, Fan Dai Di, Ma Xiao Xuan, et al. Preparation and properties of novel human-like collagen-silk fibroin scaffold for blood vessel[J]. Chinese Journal of Biotechnology, 2009, 25(8):1225-1233.
  30. Chen Jie, Wen Jian Chuan, Shi Wei hao, et al. The influence of the microtopography of silk fibroin on the proliferation of vascular endothelial cells[J]. Chinese Journal of Clinical Medicine,2013,20(3):267-269.
  31. Yashi Jin, Banani Kundu, Yurong Cai, et al. Bio-inspired mineralization of hydroxyapatite in 3D silk fibroin hydrogel for bone tissue engineering [J]. Colloids and Surfaces B: Biointerfaces, 2015,134:339-345.
  32. Bhardwaj N, Nguyen Q T, Chen A C, et al. Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering[J]. Biomaterials, 2011, 32(25):5773-5781.
  33. Sofia S, Mc Carthy M B, Gronowicz G, et al. Functionalized silkbased biomaterials for bone formation [J]. J Biomed Mater Res,2000,54(1):139-148.
  34. Meechaisue C, Wutticharoenmongkol P, Waraput R, et al. Preparation of electrospun silk fibroin fiber mats as bone scaffolds: a preliminary study [J]. Biomed Mater, 2007,2(3):181-188
  35. Zhang W J, Wang XL, Wang SY, et al. The use of injectable sonication-induced silk hydrogel for VEGF165 and BMP-2 delivery for elevation of the maxillary sinus floor[J]. Biomaterials, 2011,32(35): 9415-9424
  36. Meinel L, Fajardo R, Hofmann S, et al. Silk implants for the healing of critical size bone defects [J]. Bone,2005,37(5):688-698
  37. Hua Liu, Guo Wei Xu, Ya Fei Wang, et al. Composite scaffolds of nanohydroxyapatite and silk fibroin enhance mesenchymal stem cell-based bone regeneration via the interleukin 1 alpha autocrine/paracrine signaling loop[J]. Biomaterials,2015,49:103-112
  38. Johanna Meke, Swati Midha, Sourabh Ghosh, et al. Silk fibroin as biomaterial for bone tissue engineering, Acta Biomaterialia,2016,31:1-16
  39. Xiao Yue Sheng, Lin peng Fan, Chuang long He, et al. Vitamin E-loaded silk fibroin nanofibrous mats fabricated by green process for skin care application[J]. International Journal of Biological Macromolecules,2013,56:49-56
  40. Cai Jiang Yu, Wang Chun yang, Fan Cun Yi. Research progress of silk fibroin-containing nerve conduits in nerve tissue engineering[J]. International Journal of Orthopaedics, 2015, 36(6): 394-396.
  41. Yun Gu, Jian bin Zhu, Cheng bin Xue, et al. Chitosan/silk fibroin-based, Schwann cell-derived extracellular matrix-modified scaffolds for bridging rat sciatic nerve gaps[J]. Biomaterials, 2014,35:2253-2263.
  42. R.S. Kumar, S. Sundaresan, Gong Qing Shuan. Surgical Sutures[J]. International Textile Leader,2014,42(11):46-48.
  43. Masuhiro Takada. New Uses of Silk[J]. Journal of Agricultural, Forestry and Fisheries Technology Research, Japan,1991,14(11):3-28.
  44. Wang Yujun, Liu Xue Guang, Xu Shi qing. Development and Application of New Functions of Silkworm Silk Protein Biomaterials[J]. Silk,2006(6):44-48+50.
  45. Zhang Yuqing. Domestic Silkworm Silk Fibroin—A Natural Immobilized Enzyme Carrier[J]. Jiang Su Sericulture,1999,21(2):1-4.
  46. Grasset L, Cordier D, Ville A. Woven silk as a carrier for the immobilization of enzyme [J]. Biotech Bioeng, 1977,19(4):611-618.
  47. Jy h-Ping Chen, Chang-Ho Yang. Lipase immobilized on silk fibroin nanofibrous membrane for flavor ester synthesis[J]. Journal of Biotechnology,2008,136(4):394
  48. Fei W, Zhang Y Q. Chapter Eight Bioconjugation of Silk Fibroin Nanoparticles with Enzyme and Peptide and Their Characterization[J]. Advances in Protein Chemistry &Structural Biology, 2015,98:263-91.