Vol. 2024 No. 3 (2024)
Articles

The Influence of Polygonatum King ilium on the Activity of Calcium-Regulating Enzymes and Calcium Ion Concentration in SH-SY5Y Cells Damaged by Aβ25-35 In Vitro

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  • Yuanhua Wang,
  • Joe Zhou,
  • Junke Liu
Yuanhua Wang
Zhejiang Chinese Medical University, No. 548 Bin Wen Road, Bin Jiang District, Hangzhou City, Zhejiang Province, 310053, China.
Joe Zhou
Zhejiang Chinese Medical University, No. 548 Bin Wen Road, Bin Jiang District, Hangzhou City, Zhejiang Province, 310053, China.
Junke Liu
Zhejiang Chinese Medical University, No. 548 Bin Wen Road, Bin Jiang District, Hangzhou City, Zhejiang Province, 310053, China.
cover image

Published 02-07-2024

Keywords

  • Alzheimer's disease,
  • Polygonatum King ilium and Earthworm Formula,
  • SH-SY5Y cells,
  • Aβ25-35, calcium ions,
  • calcium ion-regulating enzymes

Copyright (c) 2024 Cambridge Science Advance

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
Y. Wang, J. Zhou, and J. Liu, “The Influence of Polygonatum King ilium on the Activity of Calcium-Regulating Enzymes and Calcium Ion Concentration in SH-SY5Y Cells Damaged by Aβ25-35 In Vitro”, Camb. Sci. Adv., vol. 2024, no. 3, pp. 22–27, Jul. 2024, doi: 10.62852/csa/2024/19.

Abstract

Objective To explore the effects of Polygonatum King ilium and Earthworm Formula (RPEWM) on the activity of calcium-regulating enzymes and free calcium ion (Ca2+) concentration in SH-SY5Y cells with Aβ damage in vitro. Methods SH-SY5Y cells were induced to differentiate with retinoic acid, then damaged by Aβ to mimic Alzheimer's disease (AD) injury. RPEWM extract was added during the AD injury to protect the cells, and its effects on cell proliferation, apoptosis, and the activity of cellular Ca2+-ATPase､Ca2+-Mg2+- ATPase ,as well as free calcium ion concentration were observed. Results RPEWM extract significantly protected cells from AD injury, resisted apoptosis, and promoted cell proliferation; it increased the activity of intracellular Ca2+-ATPase､Ca2+-Mg2+- ATPase, reduced Ca2+ concentration, and showed a dose-dependent relationship. Conclusion RPEWM has a good protective effect on differentiated SH-SY5Y cells with Aβ25-35damage and has a certain therapeutic effect on AD.

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